OSTEMER Crystal Clear resins make it possible to rapidly prototype complex lab-on-chip thanks to the two-stage curing mechanism where the liquid resin is first UV-molded into a micro-structured chip layer and then thermally cured to stiffen and bond to most substrates. Many different types of materials can also be easily integrated: filters, membranes, electrodes. No need for glue, adhesives or plasma treatment. OSTEMER Flex also enables micro patterned flexible components to be integrated on the chip.
Mixing
Mix the two components of the OSTEMER according to the proportions on the bottles in a light protected container and let stand for a few minutues to remove bubbles.
Casting
Casting OSTEMER is a good way for rapid prototyping in the lab. The mixed resin can be UV-casted and releases from most types of molds including silicones like PDMS, milled aluminum or Teflon-coated silicon/SU8 masters.
Pour the mixed resin on the mold and apply a release liner. We have found that Scotchpak 9742 PET liner from 3M has the right surface energy to easy remove the OSTEMER chip from the mold and facilitate handling. Other PET liners will work too. A standard Hg mask aligner lamp will require about 60 seconds for a complete UV-cure. If you use LED UV lamp, use 365 nm and make sure your lamp can give at least 10 mW/cm2 at 365 nm. After UV-cure demold, align to a substrate (if bonding to glass, silico or a metal, make sure it is clean) and heat cure in an oven. A temperature of 90-110 °C for 60-120 min is usually enough to completely cure and bond most designs that are a few mm thick. Wait until the device has cooled down before testing.
Properties adapted for lab-on-chip
OSTEMER 322 Crystal Clear
- Dual-cure with dry bonding using epoxy technology
- Minimal absorption of small molecules
- Minimal swelling in common solvents
- Nano-micro replication
- Optically clear
- Low autofluorescence
- Mechanically stiff
OSTEMER 324 Flex
- Dual-cure with dry bonding using epoxy technology
- Minimal absorption of small molecules
- Minimal swelling in common solvents
Nano-micro replication
Transparent
Low autofluorescence
Mechanically flexible
OSTEMER 325 Spin-on
Dual-cure with dry bonding using epoxy technology
Minimal absorption of small molecules
Minimal swelling in common solvents
Nano-micro replication
Transparent
Low autofluorescence
Mechanically stiff
Liquid resin has good wetting on silicon and glass
Selection of published articles
- Double-Sided Microwells with a Stepped Through-Hole Membrane for High-Throughput Microbial Assays (OSTEMER 322)
- Ion concentration measurement using synthetic microfluidic papers (OSTEMER 220)
- Magnetic micropump embedded in contact lens for on-demand drug delivery (OSTEMER 322)
- Rapid manufacturing of OSTE polymer RF-MEMS components (OSTEMER 322)
- Immunoassays on thiol-one synthetic paper generate a superior fluorescence signal (OSTEMER 220)
- OSTEMER polymer as a rapid packaging of electronics and microfluidic system on PCB (OSTEMER 322)
- Attaining atomic resolution from in situ data collection at room temperature using counter-diffusion-based low-cost microchips (OSTEMER 322)
- Single-Step Imprinting of Femtoliter Micro well Arrays Allows Digital Bioassays with Attomolar Limit of Detection (OSTEMER 322)
- Reaction injection molding and direct covalent bonding of OSTE+ polymer microfluidic devices (OSTEMER 322)
- Fast and inexpensive method for the fabrication of transparent pressure-resistant microfluidic chips (OSTEMER 322)
- Multi-layered, membrane-integrated microfluidics based on replica molding of a thiol–ene epoxy thermoset for organ-on-a-chip applications (OSTEMER 322)
- Rapid fabrication of OSTE+ microfluidic devices with lithographically defined hydrophobic/hydrophilic patterns and biocompatible chip sealing (OSTEMER 322)
- Dry adhesive bonding of nanoporous inorganic membranes to microfluidic devices using the OSTE(+) dual-cure polymer (OSTEMER 322)
- Exploiting nanogroove-induced cell culture anisotropy to advance in vitro brain models (OSTEMER 322)
Products recommended for lab-on-chip prototyping